- Marina Minguet Lobato: "Novel Chitinases and Hyaluronidases: Discovery and Application in the Synthesis of Bioactive Oligosaccharides"
- Angela Fernández García: "Enzymatic Biotechnology: Structure and molecular mechanisms of activity in unspecific peroxygenases for the production of compounds of chemical and pharmaceutical interest"
25 February, 2025
Two theses defended within the consortium GLICOENZ
09 September, 2024
Participation in the IV Spanish Biocatalysis Conference
"Molecular basis of specificity of a sucrose phosphorylase mutant in the glycosylation of polyphenols". M. Ruiz-Nuñez, J.L. Gonzalez-Alfonso, M. Moreno-Garcia, C. Uceda, M. Fernandez-Lobato, F.J. Plou and J. Sanz-Aparicio.
"Glucosylation of polyphenols with high yield and regioselectivity using a sucrose phosphorylase mutant". J.L. Gonzalez-Alfonso, D. Rodriguez-García, M. Moreno-García, C. Uceda-Domínguez, C. Alonso, A. Poveda, Z. Ubiparip, A.O. Ballesteros, T. Desmet, J. Jiménez-Barbero, L. Coderch, and F.J. Plou
17 August, 2024
Potential of alpha-glucosidase from Sch. occidentalis published in Appl. Microbiol. Biotechnol.
Ref: “Insights into the transglucosylation activity of α-glucosidase from Schwanniomyces occidentalis”. Zoran Merdzo, Egle Narmontaite, Jose L. Gonzalez-Alfonso, Ana Poveda, Jesus Jimenez-Barbero, Francisco J. Plou, María Fernández-Lobato. Applied Microbiology and Biotechnology 108, 443 (2024). https://doi.org/10.1007/s00253-024-13262-8
12 June, 2024
GLICOENZ at the IX National Congress of Industrial Microbiology and Microbial Biotechnology.
The GLICOENZ consortium has been widely represented in the IX National Congress of Industrial Microbiology and Microbial Biotechnology.
Laura Barahona, Noa Miguez, Egle Narmontaite, María Martínez-Ranz, Mercedes Moreno presented their works on different topics of glycoenzymes and their applications.
21 April, 2024
GLYCOENZ-GREEN meeting
In the GLYCOENZ-GREEN Project (PID2022-136367OB-C31/2/3) funded by the State Research Agency, we are studying the application of enzymes that act on carbohydrates for the production of bioactive substances (oligosaccharides and polyphenol derivatives). This week, we had a workshop at the Institute of Catalysis and Petrochemistry, along with colleagues from the Blas Cabrera Institute of Physical Chemistry and the Severo Ochoa Center for Molecular Biology, CBMSO. It was a very productive meeting where we strengthened collaborations, and where young students enthusiastically defended their work.
20 January, 2024
Publication in Microbial Cell Factories: three new chitinases from Mestchnikowia pulcherrima
Three new chitinases from M. pulcherrima, MpChit35, MpChit38 and MpChit41, were molecularly characterized and extracellularly expressed in Pichia pastoris.The three enzymes hydrolysed colloidal chitin with optimal activity at 45 ºC and pH 4.0-4.5, The partial separation and characterization of the complex COS mixtures produced from the hydrolysis of chitin and chitosan were achieved by a new anionic chromatography HPAEC-PAD method and mass spectrometry assays. An overview of the predicted structures of these proteins and their catalytic modes of action were also presented. Depicted their high sequence and structural homology, MpChit35 acted as an exo-chitinase producing di-acetyl-chitobiose from chitin while MpChit38 and MpChit41 both acted as endo-chitinases producing tri-acetyl-chitotriose as main final product.
Microbial Cell Factories 23, 31 (2024) https://doi.org/10.1186/s12934-024-02300-9
28 June, 2023
PARTICIPATION IN BIOTRANS 2023
Several members of GLICOENZ are participating the renowned Biotrans2023 congress held in La Rochelle, France. This congress offers an overview of the latest advancements in the fields of biocatalysis and biotransformations, bringing together innovative and interdisciplinary strategies to overcome scientific and technological challenges.
Marina Minguet, Fadia Cervantes, and Eglė Narmontaitė presented their work on hyaluronidases, tagatose, and fructooligosaccharides, respectively.
Marina Minguet-Lobato
Marina Minguet-Lobato, Fadia V. Cervantes, David Fernández-Polo, María Fernández-Lobato, Francisco J. Plou
06 April, 2022
Elimination of D-Glucose in carbohydrate syrups published in ACS Food Science & Technology
During the synthesis of many bioactive carbohydrates, D-glucose is released as a side-product of the transglycosylation process. It is desirable to remove it due to its caloric contribution and its effect on caries and diabetes.
04 February, 2022
The molecular machinery behind processive fungal chitinases
Ref.: “Structural inspection and protein motions modelling of a fungal glycoside hydrolase family 18 chitinase by crystallography depicts dynamic enzymatic mechanism”. E. Jiménez-Ortega, P.E. Kidibule, M. Fernández-Lobato, J. Sanz-Aparicio. Computational and Structural Biotechnology Journal 19, 5466-5478 (2021) http://doi.org/10.1016/j.csbj.2021.09.027
30 September, 2021
Thesis on exopolisaccharides producing microorganisms
Ángel García Horstmann has presented his PhD Thesis entitled "Study of moderate halophilic microorganisms productors of exopolisaccharides from Castilla-La Mancha's inland salt mines", supervised by Adrián García de Marina Bay and María Fernández Lobato (Autonomous University of Madrid, UAM). Congratulations to Ángel and his supervisors!